5. 5a. Imagine that the ARF1 GTPase protein was mutated so that it could not hydrolyze...

 

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5. 5a. Imagine that the ARF1 GTPase protein was mutated so that it could not hydrolyze GTP into GDP, regardless of its binding partners. Would you expect COPI-coated vesicles to form normally? How might subsequent or downstream steps in transport mediated by COPI-coated vesicles be affected if ARF1 cannot hydrolyze GTP into GDP? 5b. What if ARF1 protein was mutated so that it was locked in a GDP-bound state, regardless of its binding partners. How would you expect this mutation to affect COPI- coat formation? 5. 5a. Imagine that the ARF1 GTPase protein was mutated so that it could not hydrolyze GTP into GDP, regardless of its binding partners. Would you expect COPI-coated vesicles to form normally? How might subsequent or downstream steps in transport mediated by COPI-coated vesicles be affected if ARF1 cannot hydrolyze GTP into GDP? 5b. What if ARF1 protein was mutated so that it was locked in a GDP-bound state, regardless of its binding partners. How would you expect this mutation to affect COPI- coat formation?