HaeIII and BstEII restriction enzymes that cut at specificsites in the amplified sequence of interest were used.
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HaeIII and BstEII restriction enzymes that cut at specificsites in the amplified sequence of interest were used. All tubeswere kept on ice until samples were ready to incubate samples inthe Thermocycler.
Tubes #1, #2, #3 were placed at 37C for two hours. Tubes #4, #5,#6 were placed at 60C for two hours.
Make a prediction of which enzyme will cut WT vs. shy2-2vs F2. ? Question: why must the tubes be put into differenttemperatures?
For plant physiology
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