Hello can you please help me with this. Thank you very much.

You have identified a (fictional) gene C. This gene is thought to play a role in liking cold weather. You have the wild-type sequence of this gene. In talking to a coworker, you learn that he hates cold weather, so you ask to sequence his gene C. When you get the sequence back, you realize he has a mutation in this gene.

1. Based on what you know about the genetic code, would a mutation in a gene always lead to an altered protein? (Your answer should be specific to the genetic code and types of mutations that can occur in the genetic code.

Moving on, you will transcribe and translate the wild-type gene C, and then determine whether your coworkers mutation alters protein C (and therefore whether its possible that your coworker dislikes cold weather because of this mutation).

The sequence of the (fictional) gene C is below. Here, the bottom strand is the template strand. Note: The AT basepair over which the 1 of +1 is aligned represents where transcription will be begin.

+1 5...TACTAATCGA AAATTGCAGA CTGTAATGGA TTCAGAAGAT

3...ATGATTAGCT TTTAACGTCT GACATTACCT AAGTCTTCTA

GCATTAAGGT AAGT......A...TCAGG AGTAACTGAA ATAAA...

3 CGTAATTCCA TTCA......T...AGTCC TCATTGACTT TATTT...5

2. Write out the primary sequence of the RNA transcript starting at +1 and continuing until the end of the sequence

Write out the ...s as well and include the terms 5 and 3 at the appropriate positions.

Write out the sequence with the spacing i.e. same grouping of 10 bases as above where still appropriate. If youre not including the all 10 bases in a particular group, group together whatever is left over that is already grouped together as above. This is simply to make it easier for the instructor to read.

The sequence you have written out corresponds to the pre-mRNA including the 5 and 3 untranslated sequences (5 and 3 UTRs), exon 1, intron 1, and exon 2 (the last exon in the gene). Introns are spliced out of the primary transcript as part of RNA processing.

Splicing requires a 5 splice site and a 3 splice site in addition to a branch point. These sequences can be identified based on their perfect or high identity to the consensus sequences as shown below.

In the sequence you have, much of the intron 1 sequence is not given and is replaced by ....

3. Find and underline the sequence corresponding to the intron in the pre-mRNA sequence.

5'...TACTAATCGA AAATTGCAGA CTGTAATGGA TTCAGAAGAT 3'..ATGATTAGCT TTTAACGTCT GACATTACCT AAGTCTTCTA GCATTAAGGT AAGT.........TCAGG AGTAACTGAA ATAAA...3' CGTAATTCCA TTCA.........AGTCC TCATTGACTT TATTT...5