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26 Multiple Choice 1 point Which mutation would lead to no attenuateon, thus allowing for transcription and translation of the tro operon? O The 3-4 hairpin doesn't form O The 1-2 hairpin doesn't form O The 2-3 haripin doesn't form 27 Fill in the Blank 1 point Below is an operon from E. coli we haven't discussed, but has many similar features to the ones we have. The "structural genes" code for enzymes that break down arabinose (a sugar). The CAP binding site acts the same way as in the lac operon. ara02 and arall are binding sites for the araC gene product (a repressor). The big difference is that in the ABSENCE of arabinose, the repressor binds to BOTH ara02 and arall, producing a DNA loop which blocks RNA polymerase (shown in the lower figure). Another difference is that in the PRESENCE of arabinose araC the repressor acts as an activator and binds to arall and aral2 to increase polymerase activity. Under what conditions would you see expression of the araBAD structural genes? Low glucose, high arabinase choose your answer... Which of the following are true or false The CAP protein should be bound in the presence of arabinose and absence of glucose choose your answer... In the presence of arabinose araC should be bound to Arall and aral2 |choose your answer... In the absence of arabinose araC should be bound to Arall and ara02 choose your answer.. RNA polymerase DNA loop prevents RNA polymerase ara02 from binding to ParaBAD Dimeric AraC ara01 CAP aral1 aral2 ParaBAD araBAD Supresses transcription of araBAD Arabinose RNA polymerase can bind to ParaBAD CAP/CAMP RNA polymerase AraCV 3' ara02 Parac ara01 CAP aral1 aralz ParaBAD araBAD 5 ' Activates transcription of araBAD