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Problem 2: GPC to Determine Molecular Weight 12.5 points As a 1st year PhD student learning the ATRP synthesis approach, you have synthesized an atactic polystyrene polymer and would like to determine its molecular weight. You make a solution of 1 mg/mL polymer in toluene and run it on the GPC at RT. You have access to a polyisoprene calibration curve. The Mark-Houwink parameters for polyisoprene in toluene at RT are 18.6 x 10-3 ml/g and 0.74. You sample elutes at 13.5 minutes. Table 2: Polyisoprene Calibration Curve Data Molecular Weight (g/mol) 302000 220000 160000 89000 56000 35000 18000 8000 5600 Elution Time (min) 11.25 11.6 11.95 12.45 13.0 13.5 13.95 14.85 15.2 2.1 Plot the calibration curve using a log-linear plot 2.2 Determine the intrinsic viscosities of the polyisoprene standards 2.3 Plot the universal calibration curve Problem 2: GPC to Determine Molecular Weight 12.5 points As a 1st year PhD student learning the ATRP synthesis approach, you have synthesized an atactic polystyrene polymer and would like to determine its molecular weight. You make a solution of 1 mg/mL polymer in toluene and run it on the GPC at RT. You have access to a polyisoprene calibration curve. The Mark-Houwink parameters for polyisoprene in toluene at RT are 18.6 x 10-3 ml/g and 0.74. You sample elutes at 13.5 minutes. Table 2: Polyisoprene Calibration Curve Data Molecular Weight (g/mol) 302000 220000 160000 89000 56000 35000 18000 8000 5600 Elution Time (min) 11.25 11.6 11.95 12.45 13.0 13.5 13.95 14.85 15.2 2.1 Plot the calibration curve using a log-linear plot 2.2 Determine the intrinsic viscosities of the polyisoprene standards 2.3 Plot the universal calibration curve