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PROBLEM 2 The following kinetic data were obtained for an enzyme in the absence of inhibitor, and in the presence of two different inhibitors and at 5 mM concentration (you are to determine which among the data below is with and without inhibition). Assume (Eo) is the same in each experiment. (0 [2] 2 [3] [S]mM v(umol/mL.s) v(umol/mL.s) v(umol/mL.s) 1 12 4.3 5.5 2 20 8 9 4 14 13 5 35 21 16 12 40 26 18 29 a) Determine the Vm and km' for the enzyme. b) Determine the type of inhibition for the cases presented. c) Calculate the Kior K3. d) Calculate the degree of inhibition