here is a question for concentration problem.

Her is a question: when we calculate the molar absorption coefficient, shou we consider the concentration of fluorescein? given 90\muM of fluorescein. but diluted it by mixing 200\muL of fluorescein with 800\muL of phosphate buffer. So concentration of fluorescein will be 90\muM/5 (fold Calculate the absorption coefficient at the max values for each of fluorescein, NADH, fully reduced and fully oxidized cytochrome c. Use a pathlength of 1.00cm for these calculations. (Show the step of calculation) a). fluorescein: use 200L of 90M fluorescein with 800L of 40mM phosphate buffer (pH=7). Since =490nm has a max absorbance 0.87