Question: Hi , I need help. It counts as one question with different layers in our seatwork. Two proteins A and B are separated by isocratic
Hi I need help. It counts as one question with different layers in our seatwork.
Two proteins A and B are separated by isocratic elution using a cm long column, with voidage of and diameter of mm The mobile phase flow rate is mlmin The distribution coefficients for A and B are and respectively. The B peak has a characteristic peak width of minutes.
A Calculate the mobile phase retention time min
B The retention time min of protein B is
C The retention time min of protein A is
D The number of theoretical plates is
E The peak width min of protein A is
F The resolution of the separation is
G HETP cm is equal to
H If the mobile phase flow rate is increased to mlmin and the HETP is increased by determine the new number of theoretical plates.
I. The new mobile phase retention time min is
J The new retention time min for protein B is
K The new retention time min for protein A is
L The new peak width min for protein B is
M The new peak width min for protein A is
N The new resolution is
Thank you, please help.
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