Question: Hi , I need help. It counts as one question with different layers in our seatwork. Two proteins A and B are separated by isocratic

Hi, I need help. It counts as one question with different layers in our seatwork.
Two proteins A and B are separated by isocratic elution using a 50 cm long column, with voidage of 0.25 and diameter of 10 mm. The mobile phase flow rate is 10 ml/min. The distribution coefficients for A and B are 1 and 0.1 respectively. The B peak has a characteristic peak width of 0.52 minutes.
A. Calculate the mobile phase retention time (min).
B. The retention time (min) of protein B is?
C. The retention time (min) of protein A is?
D. The number of theoretical plates is?
E. The peak width (min) of protein A is?
F. The resolution of the separation is?
G. HETP (cm) is equal to?
H. If the mobile phase flow rate is increased to 20 ml/min and the HETP is increased by 80%, determine the new number of theoretical plates.
I. The new mobile phase retention time (min) is?
J. The new retention time (min) for protein B is?
K. The new retention time (min) for protein A is ?
L. The new peak width (min) for protein B is?
M. The new peak width (min) for protein A is?
N. The new resolution is?
Thank you, please help.

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