Question: hi! I need to do a graph with this data, I really need help. this is for a lab report E. READING THE RESULTS The

hi! I need to do a graph with this data, I really need help. this is for a lab report
hi! I need to do a graph with this data, I really
need help. this is for a lab report E. READING THE RESULTS
The precipitin rings will be visible in 24 to 48 hours. Carefully

E. READING THE RESULTS The precipitin rings will be visible in 24 to 48 hours. Carefully hold a plate up so that the overhead room lights shine through it. You should be able to see opaque circles around each well where antigen and antibody have precipitated. With a ruler, measure the diameter (through the centers of the wells) of the precipitin ring in millimeters. To plot the standard curve, square the diameter value and plot antigen concentration on the X-axis and the diameter squared on the Y-axis. Draw the best fit line through these points. Calculate the value of the unknown antigen concentration from this graph. Calculating your values - Use the formula: y=mx+b - This is given when you add a trendline to your graph and ask to Display equation on chart - Y= value in diameter of your sample (Ex) - X= value of concentration (what you're looking for) - So, the formula for your concentration is: - [ ] = (diameter of Ex) - b/m Standard 1.5cm 1:21.0cm. 1:40.9cm 1:80.7cm Unknown nada 1:16 nada E. READING THE RESULTS The precipitin rings will be visible in 24 to 48 hours. Carefully hold a plate up so that the overhead room lights shine through it. You should be able to see opaque circles around each well where antigen and antibody have precipitated. With a ruler, measure the diameter (through the centers of the wells) of the precipitin ring in millimeters. To plot the standard curve, square the diameter value and plot antigen concentration on the X-axis and the diameter squared on the Y-axis. Draw the best fit line through these points. Calculate the value of the unknown antigen concentration from this graph. Calculating your values - Use the formula: y=mx+b - This is given when you add a trendline to your graph and ask to Display equation on chart - Y= value in diameter of your sample (Ex) - X= value of concentration (what you're looking for) - So, the formula for your concentration is: - [ ] = (diameter of Ex) - b/m Standard 1.5cm 1:21.0cm. 1:40.9cm 1:80.7cm Unknown nada 1:16 nada

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