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You have been tasked to analysis the HMF content in a number of different honey samples. The following is the method which was carried out: The HPLC system was made up of a Waters 717 plus autosampler, a Waters 600 pump, a Waters column heater module and a Waters 996 photodiode array detector managed by Empower software (Waters Inc., Milford. MA). The stationary phase was a Symmetryn C18 analytical columx (2504.6 mm i.d.) with a particle size of 5 yun (Woodford. Sghtry Dublin). Mobile phases for elution consisted of previously degassed ultra-filtered water : acetic acid ( 99:1, v/D) (A) and methanol (B) at a constant flow rate of 0.7mL/min. The colkmn temperature was set to 30C whilst the sample chamber was set to 102C to prevent degradation of HMF. The injection volume was 20HL and the chromatograms were monitored at 285nm. A gradient elution programme was applied to first elute HMF and then switch to a less polar solvent composition to elute any remaining honey components and prevent column contamination. The gradient elution is provided in the table below: The following data represents the peak areas obtained for the samples and the calibration curve. a. Determine the average and the standard deviation. HMF content for each of the 3 different honey samples labelled. 1.5 marks b. Apply linear regression analysis and determine LOQ and LOD for the method under investigation. Please provide the software out and the workings 2 marks c. Determine the % RSD for the system|suitability and suggest whether system is stable or not. 1 mark. d. Suggest ways how we can determine the robustuness and ruggedness of this method. 2.5 mark