Question: High-Performance Liquid Chromatography I - Basic Concepts & Measurements 1. Describe the function of the basic components of a HPLC system? Name at least four.

$High-Performance Liquid Chromatography I - Basic Concepts \& Measurements 1. Describe$

High-Performance Liquid Chromatography I - Basic Concepts \& Measurements 1. Describe the function of the basic components of a HPLC system? Name at least four. 2. What is isocratic vs. gradient elution? What is a comparable technique to gradient elution that is used in GC separations? What are three benefits of gradient elution? 3. What is meant by reversed phase? Define the retention mechanism. 4. What is the purpose of a guard column? 5. What is meant by solvent strength? How would changing this affect the chromatogram? 6. How would you make peaks appear broader (take up more area)? 7. If you used cut-and-weigh or peak height for quantitative analysis, why is it important to use the same scale? 8. Name three HPLC detectors and explain their operation

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