Question: how do I calculate Km and Vmax without using excel. show steps on how to solve. 2. (20 points) Linear Regression Enzyme reaction rate data
2. (20 points) Linear Regression Enzyme reaction rate data are often fit with the Michaelis-Menten equation: VISI Km + [S] where r is the product formation rate (mmol/L/hr). V is the maximum reaction rate (mmol/L/hr), [S) is the reactant concentration (mmol/L), and K. is the reactant concentration at 50% of the maximum reaction rate (mmol/L). Please determine the values (with units) of K and a) Write the linearized form of the equation. b) Transform the data as needed. [S] (mmol/L) r(mmol/L/hr) 3.0 18.1 30 57.2 100 68.7 c) Plot the appropriate data and determine the slope and intercept of your linearized equation. d) Determine Ko and Vmar. (Include units if appropriate)
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