Question: how do I calculate Km and Vmax without using excel. show steps on how to solve. 2. (20 points) Linear Regression Enzyme reaction rate data

how do I calculate Km and Vmax without using excel. show steps on how to solve.
how do I calculate Km and Vmax without using excel. show steps

2. (20 points) Linear Regression Enzyme reaction rate data are often fit with the Michaelis-Menten equation: VISI Km + [S] where r is the product formation rate (mmol/L/hr). V is the maximum reaction rate (mmol/L/hr), [S) is the reactant concentration (mmol/L), and K. is the reactant concentration at 50% of the maximum reaction rate (mmol/L). Please determine the values (with units) of K and a) Write the linearized form of the equation. b) Transform the data as needed. [S] (mmol/L) r(mmol/L/hr) 3.0 18.1 30 57.2 100 68.7 c) Plot the appropriate data and determine the slope and intercept of your linearized equation. d) Determine Ko and Vmar. (Include units if appropriate)

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