$($i$)(10$ pts$.)$ Ray trace a fluorescence illumination path which is similar except it includes a dichroic mirror $($a flat mirror that reflects $<mn>5</mn><mn>5</mn><mn>0</mn><mi>n</mi><mi>m</mi>$ and transmits $550nm).$ Assume the lamp emits $=514nm.$ Draw or write down the locations of the field aperture and aperture diaphragm.

$($j$)(10$ pts$.)$ Find the image of a set of $4$ individual molecules in a line within the sample $($in the focal plane of the objective$)$ separated laterally by $200$ nm each, emitting $=600nm.$ Treat the molecules as emitting incoherent light. Can the molecules be resolved $($separated$)$ from one another? YES or NO

ILUMMNATION PaTH$2$

LAMP FILAMENT