I asked this question and got an answer for it. but what I don't understand is how can I get 10ml from the stock solution if I dissolved it in 2mL DI water? please see more details below

Question that I asked:

how to make a 25x stock solution if extraction buffer with 1 cOmplete tablet? Note:if we want 250 mL final amount of the solution (more info below) Preparation of Stock solution (25 X conc.): Dissolve one cOmplete Protease Inhibitor tablet in 2 ml dist. water or in 2 ml 100 mM phosphate buffer, pH 7.0. The stock solution can also be prepared in 10 mM Tris buffer, pH 7.2 to 7.5. Storage conditions (working solution): The stock solution can be stored at 2 to 8 C for 1 to 2 weeks, or at least 12 weeks at -15 to -25 C. Reconstitution Soluble in aqueous buffers, or add directly to extraction media. Alternatively, prepare 25x stock solutions in 2 ml water or 100 mM phosphate buffer, pH 7.0. Stock solution is stable for 1 to 2 weeks at 2 to 8 C or at least 12 weeks at -15 to -25 C; can also be used in thiol-containing solutions at 15 to 25 C. Answer that I got: To make a 25x stock solution, you will need to dissolve one cOmplete Protease Inhibitor tablet in 2 ml of distilled water or 100 mM phosphate buffer with pH 7.0. Here's a step-by-step procedure for preparing the 25x stock solution: Obtain a cOmplete Protease Inhibitor tablet. Choose either 2 ml of distilled water or 2 ml of 100 mM phosphate buffer with pH 7.0 to dissolve the tablet in. Put the tablet in the chosen solution and mix until it is completely dissolved. Label the solution as "25x stock solution" and store it in the refrigerator at 2 to 8 C for 1 to 2 weeks or at -15 to -25 C for at least 12 weeks. To make 250 mL of the final extraction buffer solution, you need to add 10 ml of the 25x stock solution to 240 mL of extraction buffer. To do this: Measure out 10 ml of the 25x stock solution. Measure out 240 mL of extraction buffer. Add the 10 ml of the 25x stock solution to the 240 mL of extraction buffer and mix well. Label the solution as "working solution" and use it within the recommended storage time. Note: The working solution can be stored in the refrigerator at 2 to 8 C for 1 to 2 weeks or at -15 to -25 C for at least 12 weeks. Explanation for step 1 The goal is to make a 25x stock solution of a protease inhibitor and then use it to prepare a 250 mL working solution for extraction. The 25x stock solution is made by dissolving one cOmplete Protease Inhibitor tablet in 2 ml of distilled water or 100 mM phosphate buffer with pH 7.0. The dissolved tablet will form the 25x stock solution, which can be stored in the refrigerator for 1 to 2 weeks at 2 to 8 C or for at least 12 weeks at -15 to -25 C. To prepare the 250 mL working solution, 10 ml of the 25x stock solution is added to 240 mL of extraction buffer. The final solution is mixed well and labeled as the "working solution". The working solution should be used within the recommended storage time, which is 1 to 2 weeks at 2 to 8 C or at least 12 weeks at -15 to -25 C. The reason for making a 25x stock solution is that the concentration of the protease inhibitor can be standardized, making it easier to prepare the working solution in a consistent manner. The working solution can then be used directly in the extraction process to inhibit proteolytic activity.