Question: i just need this summarized 1. Each group should provide two environmental samples (500 mL of each). One sample should be rain water. Make sure
i just need this summarized
1. Each group should provide two environmental samples (500 mL of each). One sample should be rain water. Make sure that your sample bottle is collected with no head space (completely full) and remains refrigerated until the day of the experiment. One QA/QC sample will be provided by the TA. Bring all samples to room temperature before analysis.
2. Calibrate the pH meter, if necessary. Between samples or calibration standards, you must use deionized (or distilled) water to rinse the electrode. Always catch the rinsate in a waste beaker. Always blot the probe dry with a clean Kimwipe before placing the probe in the next standard or sample. Make sure that the probe Is always submerged in liquid between readings. Do not let it get dry.
3. pH Test Strips. Take one pH test strip being careful not to touch the color squares, and dip the strip into the sample until the color no longer changes (5 seconds). Pull out the strip and immediately compare the colors. Repeat twice for all samples, and record the values in your data table in the column labeled, "pH test strip.
4. Stir each solution with a stir bar to achieve a stable reading. Follow the same procedure for rinsing and drying the probe between samples as described in step 2, and repeat for all three samples. Measure your pH values at least 2 times.
Activity 2:
1. For each of your samples, take one alkalinity test strip being careful not to touch the color squares, and dip the strip into the sample for 1 second. Pull out the strip, and compare the colors within 30 seconds (see Figure 15). This is the expected alkalinity. Make sure to record this value in your data table.
2. Select the appropriate values from Table 5. The sample volume, titration cartridge, and digit multiplier care determined from the corresponding expected alkalinity just like in Example1.1
3. Prepare the digital titrator. Slide the cartridge into the titrator receptacle and lock into position. Remove the polyethylene cap and insert a clean delivery J-tube into the end of the cartridge. Advance the plunger release button to engage the piston with the cartridge. Turn the delivery knob until air is expelled and several drops of solution flow from the tip. Then use the counter reset knob to return the digital counter back to zero, and wipe the tip before use. Start with the alkalinity OA/OC sample. Use graduated cylinder to measure the appropriate sample volume Transfer the sample into a clean beaker. Record the initial pH and temperature of the sample. Then reset the digital titrator counter to zero.
4. Add the phenolphthalein indicator. Add the contents of one phenolphthalein powder pillow to the sample. Stir to mix with the magnetic stir bar.
5. Evaluate phenolphthalein alkalinity. Make sure the delivery tube tip into the solution and swirl the flask while titrating. Record the pH readings. Record the number of digits on the titrator in a table. Mark the endpoint clearly on your table.
6. Add the bromocresol green-methyl red indicator. Add the contents of one bromocresol green-methyl red powder pillow. Stir to mix with the magnetic stir bar. The color should be blue-green.
7. Evaluate bromocresol-green alkalinity. Reset the titrator, and titrate from blue- green to the final endpoint indicated in Table 6, checking the pH with a meter. Do not over-titrate!
8. Record your pH, temperature, and color readings in a table. Regardless of the sample, if pH < 4.3, the endpoint has been reached (record your readings).
9. Plot one titration curve. For your QA/QC sample only, plot a titration curve as shown in Figure 14.
10. Repeat the titration for your other two samples. For the other two samples, you can go directly to the endpoint, without taking pH measurements and plotting a titration curve.
11. Perform all calculations to determine T, P, and BG from all your samples. The calculations should follow Example 1.2. Tabulate all your pH values, digits, and alkalinities for each sample. Note: if you forgot to reset the titrator after P alkalinity, then you will be reading the Total alkalinity (T) instead of BG alkalinity. Remember these are related by the following equation: T= P+BG
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