Question: I need help in plotting the absorbance which I obtained executing a size exclusion chromatography. I obtain the following absorbance for blue dextran fractions A

I need help in plotting the absorbance which I obtained executing a size exclusion chromatography. I obtain the following absorbance for blue dextran fractions A = 0.029, myoglobin fractions A = 0.097, and potassium chromate A = 0.097. In the process after each drop 1 mL of the fractions were collected in 14 micro text tubes. How do you express an elution profile based on the information presented? However, my doubts are which is going to be the Y and X axis, and what could be the order to begin at the zero mark. You can use excel for provide a better illustration or a table for better accuracy.

Note: Remember it must be absorbance vs elution (mL).

Additionally: Indicate the identity of each peak directly on the graph and include the max absorbance reading and absorbance wavelength for each peak.

Step by Step Solution

There are 3 Steps involved in it

1 Expert Approved Answer
Step: 1 Unlock blur-text-image
Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock
Step: 3 Unlock

Students Have Also Explored These Related Chemical Engineering Questions!