I need help to answer this questions

Capitulo 6 : link from which I have to answer the questions

https://learn-us-east-1-prod-fleet02-xythos.content.blackboardcdn.com/60e7ec5c80da0/16380633?X-Blackboard-Expiration=1651363200000&X-Blackboard-Signature=b39biNp122QpMdsc85df1pq7zzzznhdQzYBGrc%2FUWBo%3D&X-Blackboard-Client-Id=304724&response-cache-control=private%2C%20max-age%3D21600&response-content-disposition=inline%3B%20filename%2A%3DUTF-8%27%27review%2520Structural%2520proteins%2520target%2520for%2520COVID%2520presentacio%25CC%2581n%2520Biot%25204928.pdf&response-content-type=application%2Fpdf&X-Amz-Security-Token=IQoJb3JpZ2luX2VjEFIaCXVzLWVhc3QtMSJGMEQCIFbSJDd7mtVUFYJw40Lyq1NYAYvCfOaHpzD%2BBczmXWKRAiA2Gw3wL3BeBDEqsJW0Ut0mRg1Y%2FipPBJmIKmbkIiPwRirSBAgbEAIaDDYzNTU2NzkyNDE4MyIMUZZ6jHw6%2BTnbLo26Kq8EDPhnp6vjrbKRTEm2ZY3J3460qQVgH6WsdXVG0s033PCKBzL5zFsT3ntT%2BKcoLInEVdEkDgHj1fhr7vaGR207EQDwzqMeErxsJ%2BkihshDORmdDgl3%2FopLZgPNiFISnHBEyGGfQ1XJY%2FHZUbF1xY4oZFwDia5wqFqU9ReBCZdQYt97G0bU8nBQn%2BTqvW4ch0A2JIYIW2vhxZy7KzwOJcNSkagPdzi%2BJig4XNO7c%2BbIQu0ebAEC15NMWic4ZGCEgauVge6fjdvyJ%2FpO3QU01j%2BESuB4jSdZTc8TsCCJRt3fMBCzQtrsDl83RLklBYDGMEjZDCbAE29O6wIpVRCcRsbqtcTMJ%2FrarbZ8C%2BhYfQD1UPNyyjW2sa5f67NRqwRmxahd9Z0d4esxA4VWC%2BZF4IgxRZq13Pa2mHQeoXBPZyEnxDQaJcOCyEjMHrqxL%2Fs37y27qutJ94kjkgvtSoi5qvkgv154WgjJUD0LJIYi6FXQP4OW%2FTkQJdUEkWlxYLEvSRt3Tm0O1UsP8L3FAAOvKKbY8qoHPsSdZr99Whr1G91OGX0VUqcDW5GS3ymO2Bb7nyaiqNB8%2BLcIprmHUtnestIesudtsCNurDEZZDbQ3Id6yqs%2BoXemg1Ngx9u3XoYsqf%2BXOTHtkDzeATeub0wcrEhhFipc8CEquTlcGaAuipmE5JrNBpl2NcFyxLblCa8Obv8ro7PRBbdDoDS0dIsN06dW95bXSNSOEn3%2F5v%2FpZWMDlzDE77WTBjqqAR8A1so2kHfiB%2BOFmxs5CSybdNghvbAgJ4Erfy7MsXlGvUAnd4vOCO%2BSQ5EHjEThti3lalskp4P98lnEKQO6LEknrVYkJHAtbRHca7xE4%2BHq7bwnE5LtDmExd772TLqOG%2B3JbkUoH3HB0E412HOdr10%2FLVaSRhwB2Lb71%2BDb%2Bp0RGwlGIf7sceZg%2F2GNgzCLP5qECVXUFzUNLT0uO9qMgtgXChCad%2FR2FOmJ&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Date=20220430T180000Z&X-Amz-SignedHeaders=host&X-Amz-Expires=21600&X-Amz-Credential=ASIAZH6WM4PLSQGXGJN2%2F20220430%2Fus-east-1%2Fs3%2Faws4_request&X-Amz-Signature=50364440fe65b95a9644b1117a8df8519c32951f65fdeb431159549121916ae1

1. Purpose of the investigation? 2. Methods used for protein? 3. What were the researchers' goals? 4. Interpretation of results? 5. Strengths and weaknesses of the article?

Capitulo 7 : link from which I have to answer the questions

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4054378/pdf/zjv6743.pdf

1. What did they show in this study?

2. How were antibody D1-8 and HA purified?

3. How did you isolate D1-8?

4. To further characterize the functional activity of D1-8, what was done?

5. From which cell was the anti-HA monoclonal antibody (Mab) derived?

Capitulo 8 : link from which I have to answer the questions

https://learn-us-east-1-prod-fleet02-xythos.content.blackboardcdn.com/60e7ec5c80da0/16496865?X-Blackboard-Expiration=1651363200000&X-Blackboard-Signature=8ZsEj1H%2BGpXFPVF1UrbESu1QThHtUHpqZNWbs3hTgbo%3D&X-Blackboard-Client-Id=304724&response-cache-control=private%2C%20max-age%3D21600&response-content-disposition=inline%3B%20filename%2A%3DUTF-8%27%27Insulin%2520Research.pdf&response-content-type=application%2Fpdf&X-Amz-Security-Token=IQoJb3JpZ2luX2VjEFIaCXVzLWVhc3QtMSJHMEUCIEjD19KXISM83UJUQLVHVb0M3uWGgPO95a0%2BrYlIyT66AiEAhJyHCJtVFZ074Rr26Rr56LPJ73SZFZrYfn80m4u3focq0gQIGxACGgw2MzU1Njc5MjQxODMiDKrYYb%2FjBOjhLfzg2CqvBMJwZFLw5h0Y4wwYcrqkOzai%2FfGgmzYWN7yuQD4tEFrsbU7tMBQSe7s3%2Flm%2BXZ5fNfokioTzjfVkT5d5E49BV4xwlDH0%2BeM1y0vT9TELFuvsVxNgjfiC7QmYfDcEI7Xwr1X3ihPxqlyW8qw8jHvRJ0sT8G4hTCpdDz39TldomvodUeK8AMUiSfH2dF4JR8gjRwTo%2FAfphC%2BNLv727h4yEX3ehfsxmDMowa6%2BxakKIvgCysNJ0cOCRcqQQ%2FDaAqfyVbyZjbGq0WRX2T9kuHsG68ilv1IrPrm3FjNePeLQD4hKnKiiJJwobh6MpF%2BuxszSSQuw0ue4EjgDf6IHtFG4Ds7XmFI6hqalsqJ7D%2BYR9zALiVsfj6YM3a7paip2makL0kZFdJyhEa3wyg0QmBImb6OGS3Y0ERPFSAlGl0ci26s9MVkGKTEYks4IkO13GlZh6Jsv3hp3GWTyH6L1liDvPIbBgawiSdcx5Rw4GTl%2Fhz3d6IIYZVZMOQ7j4cCf%2FL5gVpLQtKYMSBPhmKpxh4RPjW6shwEBELbZ0GrKXNqxy6gEY7OGr%2B%2FOyFQe1yma6iDjzzQ%2FBvxxa3hI5RaHdHH302KlCFFBOid4GvFQspDvLW1xuyiAyWQ2TgTLAfupaSYVVo5pu5XxP2OYE6J%2FGPf6pEvQgxMCdXKEEjLj%2BYLe9SUnjUBLR%2FkFwbW1X3dVQWv0BcP%2FbjrdQZaAxprkBRTDFRZ9sc%2BHS6Puq%2FYW2SmBohUwpvG1kwY6qQED4PGaBSnRUXk%2Bw3uweUEaYRpZYIgnOwkJ7ryEP5Ed9AT42JOpLfEgS4rNG8nzONZG6t1o5l53yngLli4T5GTwOqfgI3R75kbZM1%2BSjIiLUBRqRUL4VMc3JAefC5MWzvp1fr6Y%2BALBt5J5EVSN3jyMHkJRLmBYx21fZ6lSqmPeW1Z8R8VaXsNQXssP7RjvFDuiC2mF1AOupJqiM01UWlXFvIIOt%2FXzqS1G&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Date=20220430T180000Z&X-Amz-SignedHeaders=host&X-Amz-Expires=21600&X-Amz-Credential=ASIAZH6WM4PLQM3IBH3T%2F20220430%2Fus-east-1%2Fs3%2Faws4_request&X-Amz-Signature=7cc7d0a4cd2ce78d95b10c667be9031baf731e7f34bf01a0b3490a499e564b6d

1. What was the purpose of the investigation?

2. What did they build? How were chains A and B released? How did they purify the protein?

3. What did you want to achieve with B-Cry/A chain (B-AC) and B-Cry/B chain (B-BC)?

4. What did the oligomerization studies and in vivo activity show?

5. Why is B-Cry suggested?