Question: i want to know whether there is significant difference between two samples in the dataset. one population size is 500 and second population size is

i want to know whether there is significant difference between two samples in the dataset.

one population size is 500 and second population size is 250. i will include a picture

the dataset is( https://www.kaggle.com/uciml/pima-indians-diabetes-database)

i want to know whether there is significant difference between two samples

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