If pyruvate oxidation is blocked, what will happen to the levels of oxaloacetate and citric acid...

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If pyruvate oxidation is blocked, what will happen to the levels of oxaloacetate and citric acid in the citric acid cycle? Note: oxaloacetate is the 4-carbon molecule produced in the eighth step of the citric acid cycle. Oxaloacetate and the acetyl group combine to form citric acid in the first step of the citri acid cycle. A) There will be no change in the levels of oxaloacetate and citric acid. B) Oxaloacetate will decrease and citric acid will accumulate. C) Oxaloacetate will accumulate and citric acid will decrease. D) Both oxaloacetate and citric acid will decrease. 8. You briefly expose bacteria undergoing DNA replication to radioactively labeled nucleotides. When You centrifuge the DNA isolated from the bacteria, the DNA separates into two classes. One class of labeled DNA includes very large molecules (thousands or even millions of nucleotides long), and the other includes short stretches of DNA (several hundred to a few thousand nucleotides in length). These two classes of DNA probably represent A) lagging strands and Okazaki fragments. (B)) leading strands and Okazaki fragments. C) Okazaki fragments and RNA primers. D) leading strands and RNA primers. 9. Allolactose, an isomer of lactose, is formed in small amounts from lactose. An E. coli cell is presented for the first time with the sugar lactose (containing allolactose) as a potential food source. Which of the following occurs when the lactose enters the cell? A) The lac repressor protein attaches to the operator. B) Allolactose binds to the operator. Allolactose binds to the lac repressor protein. D) The repressor protein and allolactose bind to RNA polymerase. 10. A couple of students in BIOE 120 decided to "replicate" Meselson and Stahl's experiment with a small modification. They grew bacteria in media containing N for a couple of generations. Then they transferred these bacteria to media containing "N. After one generation, they extracted the DNA. After DNA extraction, they heated the DNA to 94°C and used a salt gradient and centrifugation to separate the molecules. What will their centrifugation tube look like after one round of replication in 15N media, extraction, heating, and centrifugation for an organism that replicates semi- conservatively? Control tube B) A) C) D) If pyruvate oxidation is blocked, what will happen to the levels of oxaloacetate and citric acid in the citric acid cycle? Note: oxaloacetate is the 4-carbon molecule produced in the eighth step of the citric acid cycle. Oxaloacetate and the acetyl group combine to form citric acid in the first step of the citri acid cycle. A) There will be no change in the levels of oxaloacetate and citric acid. B) Oxaloacetate will decrease and citric acid will accumulate. C) Oxaloacetate will accumulate and citric acid will decrease. D) Both oxaloacetate and citric acid will decrease. 8. You briefly expose bacteria undergoing DNA replication to radioactively labeled nucleotides. When You centrifuge the DNA isolated from the bacteria, the DNA separates into two classes. One class of labeled DNA includes very large molecules (thousands or even millions of nucleotides long), and the other includes short stretches of DNA (several hundred to a few thousand nucleotides in length). These two classes of DNA probably represent A) lagging strands and Okazaki fragments. (B)) leading strands and Okazaki fragments. C) Okazaki fragments and RNA primers. D) leading strands and RNA primers. 9. Allolactose, an isomer of lactose, is formed in small amounts from lactose. An E. coli cell is presented for the first time with the sugar lactose (containing allolactose) as a potential food source. Which of the following occurs when the lactose enters the cell? A) The lac repressor protein attaches to the operator. B) Allolactose binds to the operator. Allolactose binds to the lac repressor protein. D) The repressor protein and allolactose bind to RNA polymerase. 10. A couple of students in BIOE 120 decided to "replicate" Meselson and Stahl's experiment with a small modification. They grew bacteria in media containing N for a couple of generations. Then they transferred these bacteria to media containing "N. After one generation, they extracted the DNA. After DNA extraction, they heated the DNA to 94°C and used a salt gradient and centrifugation to separate the molecules. What will their centrifugation tube look like after one round of replication in 15N media, extraction, heating, and centrifugation for an organism that replicates semi- conservatively? Control tube B) A) C) D)

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