If the culture volume loading (working volume) is about 80% of the total fermenter tank volume, design the fermenter tank to produce 10kg purified EPO/year step by step by answering the followings:

1)What is the total EPO (kg) required from fermentation to produce 10 kg purified EPO by purification?

2)What is the total fermentation volume/year to produce 10 kg purified EPO by batch and by chemostat individually?

3)If batch mode is operated, how many batches can be operated per year? What is the fermentation volume per batch?

4)If chemostat continuous mode is operated by continuous feeding and continuous harvesting, What is the culture volume in the fermenter in chemostat continuous fermentation to product 10kg purified EPO? What is the fermenter tank volume applied in chemostat?

5)By comparing batch and continuous chemostat fermentation fermenter volume and design, what would you recommend regarding the selection of operation mode of upstream process? Why?
 

Transcribed Image Text:

You are assigned to design a manufacturing plant to produce the biosimilar of EPO (Recombinant Human Erythropoietin) in the Asian Pacific region. The total annual production is 10Kg with the purity (up to 99%) purity and quality meet the FDA requirement. The following information are given: Cell line: genetic modified CHO cell line or other recombinant organisms, serum-free medium in fermentation if choose CHO cell line to produce EPO Fast flow agarose absorbents will be used in the chromatography in purification process The following block flow diagram is applied to produce EPO Recovery 80% Recovery 90% Recovery 60% Hydrophobic Size Exclusion lon-Exchange Chromatography Fermentation interaction Chromatography Chromatography 10kg purified Centrifugation Ultrafiltration ЕРО Recovery 95% Recovery 95% You are assigned to design a manufacturing plant to produce the biosimilar of EPO (Recombinant Human Erythropoietin) in the Asian Pacific region. The total annual production is 10Kg with the purity (up to 99%) purity and quality meet the FDA requirement. The following information are given: Cell line: genetic modified CHO cell line or other recombinant organisms, serum-free medium in fermentation if choose CHO cell line to produce EPO Fast flow agarose absorbents will be used in the chromatography in purification process The following block flow diagram is applied to produce EPO Recovery 80% Recovery 90% Recovery 60% Hydrophobic Size Exclusion lon-Exchange Chromatography Fermentation interaction Chromatography Chromatography 10kg purified Centrifugation Ultrafiltration ЕРО Recovery 95% Recovery 95%

Answer rating: 100% (QA)

1 2565 kgyr EPO from the fermenter 2 Batch 4873294 Lyr Continuous Chemostat 2436647 Lyr 3 Number of Batches 40 batchyr Fermetation Volume 121832 Lyr 4 Culture Volume 56855 L Tank Volume 71069 L 5 Cont... View the full answer