Question: In a Phase Contrast Microscopy set-up the lens used for Fourier transform has a diameter of 5 cm and the wavelength used is 500 nm.

In a Phase Contrast Microscopy set-up the lens used for Fourier transform has a diameter of 5 cm and the wavelength used is 500 nm. What should be the size and thickness of the circular spot on a glass plate to be used as the filter, if the material used is MgF2 with an index of 1.38?

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