Question: In F ca you explain the answer Now, you prepare the plasmid, pBIO486 for transformation: The tube containing the plasmid is labeled, OD 260nm =

In F ca you explain the answer

Now, you prepare the plasmid, pBIO486 for transformation: The tube containing the plasmid is labeled, "OD 260nm = 0.470"; that is, (0.47)(50ug/ml) = 23.5 ug/ml it has an absorbance of 0.47, as is. E. What is the concentration of DNA in the tube? You would like to use 35ng of the plasmid in a single transformation. (35 ng) x (lul/23.5ng) = 1.49 ul F. What volume of plasmid should you add to the competent cells? (use 1.5 ul) Express your answer in microliters (ul)

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