Question: In Lab D, you will be using a technique called thin layer chromatography (TLC) to analyze the identities and purities of various samples. To prepare
In Lab D, you will be using a technique called thin layer chromatography (TLC) to analyze the identities and purities of various samples. To prepare a TLC plate for analysis, you will use a capillary spotter to apply small spots of each compound/mixture dissolved in a volatile solvent onto the spotting line (AKA the "origin"). The spotting line should be a pencil line drawn about 1 cm above the bottom of the plate. Sandy Student is new to TLC, so they made several mistakes during Lab D: Sandy spotted the analytes onto the plate seven times each. This is not a good idea because: [ Select ] Sandy got a new TLC plate and started over again. They spotted an intermediate amount of the first analyte onto the plate but, getting impatient now, forgot to drain any remaining sample left behind in the spotter onto a paper towel and rinse the spotter with acetone before spotting the second analyte. This would be a problem because: [ Select ]
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