Question: In order to clone a sequence into a plasmid vector, the vector must contain certain sequences. a) What sequence is essential so that a plasmid

In order to clone a sequence into a plasmid vector, the vector must contain certain sequences. a) What sequence is essential so that a plasmid vector will be maintained in the cells as they grow and divide? (1 mark) b) What sequence is required if the aim is to express the gene in the cells into which it is cloned? (1 mark) c) What sort of sequence allows for the selection of only those bacteria that contain the plasmid? (1 mark) d) What sort of sequence allows for recombination with insert DNA? (1 mark) You want to clone a gene from some giraffe cells so that you can express the protein in E. coli using a plasmid vector. (e) Outline the steps you need to go through to clone the giraffe gene into the plasmid vector so that you can grow a culture of E. coli cells that will express this protein

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