Question: In this experiment, you will learn how to use a apeetrophetrmeter to eal. culate concentrations, using Beer's law. You will also doy a simple prutein

 In this experiment, you will learn how to use a apeetrophetrmeter

In this experiment, you will learn how to use a apeetrophetrmeter to eal. culate concentrations, using Beer's law. You will also doy a simple prutein assay and learn to create standard curves for the determination of the protein concentration in an unkrown. 1. If your spectrophotometer can measure an (absorbance up to 1.5) what is the maximum concentration of NADH that you can measure with out diluting? 2. If you add 3mL of water to 1mL of NADH, mix and get an absorbance of 0.2, what is the concentration of the original NADH solution? 3. What size test tubes will you use to mix the reagents for the Bradford protein assay

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