Question: Integration Host Factor, IHF, is an architectural protein in E. coli that helps to package the DNA inside the cell as well as to organize

Integration Host Factor, IHF, is an architectural protein in E. coli that helps to package the DNA inside the cell as well as to organize the DNA for higher-order nucleoprotein complexes. IHF also binds to specific sites on the DNA that are 35 base-pairs (bps) long and bends that DNA at that site by rad (180). The Young's modulus of DNA is 300 MPa, and its cross-sectional radius is 1 nm. Model DNA as a cylindrical rod. A. What is the bending coefficient of DNA in units of J m? Express your answer in scientific notation (For example 6.02 1023 as 6.02 23.) B. How long is a 35-bp segment of DNA? (Express your answer in nm; assume that the separation between bps is 0.34 nm.) C. Determine the radius of the curvature (in nm) for the DNA in the IHF-bound bent complex. D. Calculate the energy (in multiples of ) required to bend the DNA, where is body temperature (98.6 degrees Fahrenheit).

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