Intestinal Uptake of Leucine You are studying the uptake of L-leucine by epithelial cells of the...
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Intestinal Uptake of Leucine You are studying the uptake of L-leucine by epithelial cells of the mouse intestine. Measurements of the rate of uptake of L-leucine and several of its analogs, with and without Na+ in the assay buffer, yield the results given in the table. What can you conclude about the properties and mechanism of the leucine transporter? Would you expect L-leucine uptake to be inhibited by ouabain? Substrate L-Leucine D-Leucine L-Valine Uptake in presence of Na+ Vmax K (mm) 420 310 225 0.24 4.7 0.31 Uptake in absence of Na+ Vmax K (mm) 23 5 19 0.2 4.7 0.31 Intestinal Uptake of Leucine You are studying the uptake of L-leucine by epithelial cells of the mouse intestine. Measurements of the rate of uptake of L-leucine and several of its analogs, with and without Na+ in the assay buffer, yield the results given in the table. What can you conclude about the properties and mechanism of the leucine transporter? Would you expect L-leucine uptake to be inhibited by ouabain? Substrate L-Leucine D-Leucine L-Valine Uptake in presence of Na+ Vmax K (mm) 420 310 225 0.24 4.7 0.31 Uptake in absence of Na+ Vmax K (mm) 23 5 19 0.2 4.7 0.31
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