Lab result: https://youtu.be/gYcLqlTLFz0

Lab result: https://youtu.be/Kb7HrI4saQ0

2. What was the purpose of the (-) plasmid on the TSA plate? B) What was the purpose of the (+) plasmid on the TSA plate? 3 Why was it necessary to include ampicillin in the medium with the (-) plasmid treatment? B) Why was it necessary to include ampicillin in the medium with the (+) plasmid treatment? 4 Why were the two genes of interest on the plasmid only expressed on the plate with ampicillin? 5 What causes the "halo effect", the white satellite colonies around the green fluorescent colonies, in the (+) plasmid TSA + ampicillin plate? 6 Other than transformation, what are two other mechanisms that bacteria use to acquire new genes? Please describe them in your own words. A-HA! Moment or Muddiest Point: Describe your A-HA! Moment either the lab procedure itself or during the observation phase of this lab (i.e., describe something new, interesting, or unexpected that you learned in lab today). Or, describe the Muddiest Point (i.e., describe what was the most confusing or difficult component of this lab).