Lontoh and Semrau (1998) examined the effect of the copper concentration on the rate of methane...

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Lontoh and Semrau (1998) examined the effect of the copper concentration on the rate of methane (CH.) degradation by Methylosinus trichosporium OB36. A set of data from their work is tabulated below right. The rate of methane degradation can be described using the Monod model, i.e. Effect of copper concentration on methane degradation rate ĝs K +S Cu = 2.5 umol/L Cu- 20 μmol/L Where S is the substrate (methane) 1.0 6.38 1.0 6.38 concentration, q is the specific substrate degradation rate, q is the maximum specific substrate degradation rate, and K is the half-saturation coefficient. Model 5.0 23.40 5.0 23.40 55.74 10.7 10.4 49.36 20.5 74.47 66.81 20.2 30.9 110.21 30.9 71.49 parameters can be estimated by plotting 67.66 48.8 149.79 48.8 the Lineweaver-Burk transformation of 167.23 73,19 the model, i.e. 81.1 81.5 1 K1 1 Units: S, umol CH/L; q, umol CHa/mg protein-min. a. Determine the Monod parameters q and K methane degradation at each copper concentration. Include the Lineweaver-Burk plots in your submission. Also plot the model against the data points on q vs. S coordinates. b. Determine the type of inhibition exerted by copper on methane degradation. Also determine the inhibition coefficient. (Note that, if the inhibition mechanism affects both q and K, it is possible for a different inhibition coefficient to apply to each parameter. Reference: Lontoh S, Semrau JD (1998). Methane and Trichloroethylene Degradation by Methylosinus trichosporium OB36 Expressing Particulate Methane Monooxygenase. Applied and Environmental Microbiology 64(3), 1106-1114. Lontoh and Semrau (1998) examined the effect of the copper concentration on the rate of methane (CH.) degradation by Methylosinus trichosporium OB36. A set of data from their work is tabulated below right. The rate of methane degradation can be described using the Monod model, i.e. Effect of copper concentration on methane degradation rate ĝs K +S Cu = 2.5 umol/L Cu- 20 μmol/L Where S is the substrate (methane) 1.0 6.38 1.0 6.38 concentration, q is the specific substrate degradation rate, q is the maximum specific substrate degradation rate, and K is the half-saturation coefficient. Model 5.0 23.40 5.0 23.40 55.74 10.7 10.4 49.36 20.5 74.47 66.81 20.2 30.9 110.21 30.9 71.49 parameters can be estimated by plotting 67.66 48.8 149.79 48.8 the Lineweaver-Burk transformation of 167.23 73,19 the model, i.e. 81.1 81.5 1 K1 1 Units: S, umol CH/L; q, umol CHa/mg protein-min. a. Determine the Monod parameters q and K methane degradation at each copper concentration. Include the Lineweaver-Burk plots in your submission. Also plot the model against the data points on q vs. S coordinates. b. Determine the type of inhibition exerted by copper on methane degradation. Also determine the inhibition coefficient. (Note that, if the inhibition mechanism affects both q and K, it is possible for a different inhibition coefficient to apply to each parameter. Reference: Lontoh S, Semrau JD (1998). Methane and Trichloroethylene Degradation by Methylosinus trichosporium OB36 Expressing Particulate Methane Monooxygenase. Applied and Environmental Microbiology 64(3), 1106-1114.

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