MATH SOL 6. Solved to an extent Inhibition type and parameters The inhibitive effect of maltose and a-dextrin on the enzyme-catalysed hydrolysis of starch (S) has been studied in a laboratory chemostat. Three sets of experiments have been performed: a) no inhibitor Cs [g/L] r (relative) 12.5 100 9.0 92 4.25 70 1.0 29 b) (maltose) = 12.7 mg/L Cs [g/L] r (relative) 10.0 77 5.25 62 2.0 38 1.67 34 c) [a-dextrin) = 3.34 mg/L Cs [g/L] r (relative) 33.0 116 10.2 85 3.6 55 1.6 32 The measured rates are relative to a base case (no inhibition, 12.5 g/L starch is assumed to be 100 units). If you solve by hand or in Excel, use graphical approach or the formulae (4-12). If you solve using Maple, I expect to see a non-linear fit! a) Determine the maximum reaction rate ke and the Michaelis constant KM in the absence of inhibition. b) and c) Determine the type of inhibition for the two inhibitors, and the respective constants K. = intercept xy-x.xy - = 0.75 s/M, slope = = 0.021 s. x - x x - x x (4-12) -2 -2 - 17