Question: Method/process How does it impact the POI? Altering the origin of replication on the plasmid Altering the promoter Deleting the RBS of the antibiotic resistance

Method/process How does it impact the POI? Altering the origin of

Method/process How does it impact the POI? Altering the origin of replication on the plasmid Altering the promoter Deleting the RBS of the antibiotic resistance gene Codon optimizing the DNA sequence of the GOI Adding a leader sequence to GOI Adding a purification tag to GOI Co-expressing a chaperone in cells

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