Microtubules are filamentous structures in cells that maintain cell shape and facilitate the movement of molecules within the cell. They are long, hollow cylinders with a diameter of about 25 nm. It is possible to incorporate fluorescent molecules into microtubules when illuminated by ultraviolet light, the fluorescent molecules emit visible light that can be imaged by the optical system of a microscope.

If the emitted light has a wavelength of 450 nm and the NA of the microscope objective is 1.2, what is the smallest resolvable separation between two objects in the microscope?

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To find the smallest resolvable separation between two objects in the microscope we c... View the full answer