my question is how to create an ANOVA using this data. can someone explain how i were to go about that

hi, so the data is gathered from an elodea plant submerged in dark, shaded, and clear at 10 and 25 degrees celcius. two groups had differing results for final temperatures, initial; and final and inital change in o2

attached is the data for both group's data.

i am trying to figure out the anova of this data.

290 nia na nia C D E F G H I Enter data in all shaded cells Group # Friday, Actual Incubator Temp: Actual Incubator Temp: 2:00pm Group 2 10 10 10- 10 25 25- 25- 25- initial dark shaded light initiat dark shaded light Initial Dissolved Oxygen 9.63 9.63 9.82 7.85 Bottle Temperature 11.10 10.80 11.20 na 22.20 22.20 22.20 11 Elodea wt 1.79 2.31 2.22 nia 2.04 2.22 2 Incubation start time 3:30 3:30 3:30 Enta 3 Incubation stop time 2:57 2:49 2:44 na 2:30 4 Total incubation time fhrs) na 167.45 167.32' 167.23 nia 167.00 167.07 167.13 End temperature 13.20 14.80 13.50 21.60225022.50 5 End O, mg/l 9.69 0.14 3.11 3.517.880.151.15 16.60 6 0; change (as mg/L) nia 9.49 -6.52 -6.31 nia -7.75 -6.73 8 .75 7 Bottle vol in L na 0.30 0.300.31nia 0.31 0.300.31 O, change/bottle nia -2.88 -1.98 -1.92 n ia 2.36 2.04 2.68 90, changeigm nta -1.61 -0.86 0.87 na -1.04 -1.00 1.21 10 Ostgmit nia -0.01 -0.01 0.01 nia -0.01 -0.01 0.01 11 grosso, change na 0.00 0.00 0.00 na 0.00 12 gross prim prod 0.00 0.01 na 0.00 3.50 lugms glucose iam ih 3.46 nia 0.00 0.20 10.47 nra & & 10 low 10 High &&&& F_1100_1 F_1100_2 F_1400_1 F_1400_2 EXAMPLE Alt format n/a n/a n/a Enter data in all shaded cells Group # Friday, 2:00pm Actual Incubator Temp: Group 1 100 100 100 initial dark shaded Initial Dissolved Oxygen 9.55 9.60 Bottle Temperature 11.80 11.20 1 Elodea wt n/a 1.70 1.99 2 Incubation start time 3:25 3:25 3 Incubation stop time n/a 2:53 2:47 4 Total incubation time (hrs) n/a 167.47 167.37 End temperature 9.70 13.10 5 End O2 mg/L 9.58 0.16 3.31 6 02 change (as mg/L) -9.39 -6.29 7 Bottle vol in L 0.30 0.30 8 02 change/bottle n/a -2.82 -1.89 9 02 change/gm n/a -1.66 -0.95 10 O2/gm/hr -0.01 -0.01 11 gross 02 change 0.00 0.00 12 gross prim prod n/a 0.00 3.29 (ugms glucose/gm/hr) 100 light 9.60 11.20 0.94 3:25 2:40 167.25 12.80 5.80 -3.80 0.30 -1.14 -1.21 -0.01 0.00 2.06 Actual Incubator Temp: 250 250 250 initial dark shaded 7.92 7.90 n/a 22.30 22.70 n/a 1.94 1.68 n/a 3:18 3:18 2:24 2:28 n/a 167.10 167.17 21.80 23.00 7.94 0.11 1.75 n/a -7.81 -6.15 n/a 0.30 0.30 n/a -2.34 -1.83 n/a -1.21 -1.09 n/a -0.01 -0.01 n/a 0.00 0.00 n/a 0.00 0.56 250 light 8.00 22.00 2.23 3:18 2:32 167.23 22.30 14.18 6.18 0.28 1.73 0.78 0.00 0.01 9.23 n/a n/a n/a n/a 10-Low 10-High 3 2 25-Low 25-High F_1100_1 F_1100_2 F_1400_1 F_1400_2 Alt format 250 250 light 7.85 22.20 2.22 3:22 2:30 Enter data in all shaded cells Group # Friday, 2:00pm Actual Incubator Temp: Group 2 100 100 100 initial dark shaded Initial Dissolved Oxygen 9.63 9.63 Bottle Temperature n/a 11.10 10.80 1 Elodea wt n/a 1.79 2.31 2 Incubation start time n/a 3:30 3:30 3 Incubation stop time n/a 2:57 2:49 4 Total incubation time (hrs) n/a 167.45 167.32 End temperature 13.20 14.80 5 End O2 mg/L 9.69 0.14 3.11 6 O2 change (as mg/L) n/a -9.49 6.52 7 Bottle vol in L n/a 0.30 0.30 8 O2 change/bottle n/a -2.88 -1.98 9 02 change/gm n/a -1.61 -0.86 10 O2/gm/hr n/a -0.01 -0.01 11 gross O2 change n/a 0.00 0.00 12 gross prim prod n/a 0.00 3.50 (ugms glucose/gm/hr) 100 light 9.82 11.20 2.22 3:30 2:44 167.23 13.50 3.51 -6.31 0.31 -1.92 -0.87 -0.01 0.00 3.46 Actual Incubator Temp: 250 250 initial dark shaded 7.90 7.88 n/a 22.20 22.20 n/a 2.27 2.04 n/a 3:22 3:22 n/a 2:22 2:26 n/a 167.00 167.07 21.60 22.50 7.88 0.15 1.15 n/a -7.75 -6.73 0.31 0.30 n/a -2.36 -2.04 n/a -1.04 -1.00 -0.01 -0.01 n/a 0.00 0.00 n/a 0.00 0.20 n/a 167.13 22.50 16.60 8.75 0.31 2.68 1.21 0.01 0.01 10.47 n/a 10-Low 10-High 25-Low 25-High 290 nia na nia C D E F G H I Enter data in all shaded cells Group # Friday, Actual Incubator Temp: Actual Incubator Temp: 2:00pm Group 2 10 10 10- 10 25 25- 25- 25- initial dark shaded light initiat dark shaded light Initial Dissolved Oxygen 9.63 9.63 9.82 7.85 Bottle Temperature 11.10 10.80 11.20 na 22.20 22.20 22.20 11 Elodea wt 1.79 2.31 2.22 nia 2.04 2.22 2 Incubation start time 3:30 3:30 3:30 Enta 3 Incubation stop time 2:57 2:49 2:44 na 2:30 4 Total incubation time fhrs) na 167.45 167.32' 167.23 nia 167.00 167.07 167.13 End temperature 13.20 14.80 13.50 21.60225022.50 5 End O, mg/l 9.69 0.14 3.11 3.517.880.151.15 16.60 6 0; change (as mg/L) nia 9.49 -6.52 -6.31 nia -7.75 -6.73 8 .75 7 Bottle vol in L na 0.30 0.300.31nia 0.31 0.300.31 O, change/bottle nia -2.88 -1.98 -1.92 n ia 2.36 2.04 2.68 90, changeigm nta -1.61 -0.86 0.87 na -1.04 -1.00 1.21 10 Ostgmit nia -0.01 -0.01 0.01 nia -0.01 -0.01 0.01 11 grosso, change na 0.00 0.00 0.00 na 0.00 12 gross prim prod 0.00 0.01 na 0.00 3.50 lugms glucose iam ih 3.46 nia 0.00 0.20 10.47 nra & & 10 low 10 High &&&& F_1100_1 F_1100_2 F_1400_1 F_1400_2 EXAMPLE Alt format n/a n/a n/a Enter data in all shaded cells Group # Friday, 2:00pm Actual Incubator Temp: Group 1 100 100 100 initial dark shaded Initial Dissolved Oxygen 9.55 9.60 Bottle Temperature 11.80 11.20 1 Elodea wt n/a 1.70 1.99 2 Incubation start time 3:25 3:25 3 Incubation stop time n/a 2:53 2:47 4 Total incubation time (hrs) n/a 167.47 167.37 End temperature 9.70 13.10 5 End O2 mg/L 9.58 0.16 3.31 6 02 change (as mg/L) -9.39 -6.29 7 Bottle vol in L 0.30 0.30 8 02 change/bottle n/a -2.82 -1.89 9 02 change/gm n/a -1.66 -0.95 10 O2/gm/hr -0.01 -0.01 11 gross 02 change 0.00 0.00 12 gross prim prod n/a 0.00 3.29 (ugms glucose/gm/hr) 100 light 9.60 11.20 0.94 3:25 2:40 167.25 12.80 5.80 -3.80 0.30 -1.14 -1.21 -0.01 0.00 2.06 Actual Incubator Temp: 250 250 250 initial dark shaded 7.92 7.90 n/a 22.30 22.70 n/a 1.94 1.68 n/a 3:18 3:18 2:24 2:28 n/a 167.10 167.17 21.80 23.00 7.94 0.11 1.75 n/a -7.81 -6.15 n/a 0.30 0.30 n/a -2.34 -1.83 n/a -1.21 -1.09 n/a -0.01 -0.01 n/a 0.00 0.00 n/a 0.00 0.56 250 light 8.00 22.00 2.23 3:18 2:32 167.23 22.30 14.18 6.18 0.28 1.73 0.78 0.00 0.01 9.23 n/a n/a n/a n/a 10-Low 10-High 3 2 25-Low 25-High F_1100_1 F_1100_2 F_1400_1 F_1400_2 Alt format 250 250 light 7.85 22.20 2.22 3:22 2:30 Enter data in all shaded cells Group # Friday, 2:00pm Actual Incubator Temp: Group 2 100 100 100 initial dark shaded Initial Dissolved Oxygen 9.63 9.63 Bottle Temperature n/a 11.10 10.80 1 Elodea wt n/a 1.79 2.31 2 Incubation start time n/a 3:30 3:30 3 Incubation stop time n/a 2:57 2:49 4 Total incubation time (hrs) n/a 167.45 167.32 End temperature 13.20 14.80 5 End O2 mg/L 9.69 0.14 3.11 6 O2 change (as mg/L) n/a -9.49 6.52 7 Bottle vol in L n/a 0.30 0.30 8 O2 change/bottle n/a -2.88 -1.98 9 02 change/gm n/a -1.61 -0.86 10 O2/gm/hr n/a -0.01 -0.01 11 gross O2 change n/a 0.00 0.00 12 gross prim prod n/a 0.00 3.50 (ugms glucose/gm/hr) 100 light 9.82 11.20 2.22 3:30 2:44 167.23 13.50 3.51 -6.31 0.31 -1.92 -0.87 -0.01 0.00 3.46 Actual Incubator Temp: 250 250 initial dark shaded 7.90 7.88 n/a 22.20 22.20 n/a 2.27 2.04 n/a 3:22 3:22 n/a 2:22 2:26 n/a 167.00 167.07 21.60 22.50 7.88 0.15 1.15 n/a -7.75 -6.73 0.31 0.30 n/a -2.36 -2.04 n/a -1.04 -1.00 -0.01 -0.01 n/a 0.00 0.00 n/a 0.00 0.20 n/a 167.13 22.50 16.60 8.75 0.31 2.68 1.21 0.01 0.01 10.47 n/a 10-Low 10-High 25-Low 25-High