my variable is Nacl concentration. i have 4 test tubes totalling to 10ml. the tubes will have 0%, 2.5%, 5%, and 10% NaCl concentration. i need help figuring out how much spg buffer thats needed in each test tube and knowing how much 5M NaCl to put in.

Design an experiment that will test your variable, you will need: lactase, lactose, a buffer, and potentially the variable you are testing (if it is something like Naci concentration). You need to use the CIV1=C2V2 equation to determine the amount o ...r colution. look for the parameters in the lab manual. What is available? 6% Lactose solution Goat Milk Cow Milk Lactase enzyme 1 M SPG buffer pH 4 and pH 10 water baths 0,20, 40, 60, 80 C 5M NaCl - Dish soap - deionized water Sucrose Maltose 6% Glucose What do you want in your final solutions? 52% lactose 0.1-0.2 M SPG buffer- for a consistent pH 5-15 ml total volume 0.5-1 ml enzyme solution (pill dissolved in 50 mls H20) a Design an experiment that will test your variable, you will need: lactase, lactose, a buffer, and potentially the variable you are testing (if it is something like Naci concentration). You need to use the CIV1=C2V2 equation to determine the amount o ...r colution. look for the parameters in the lab manual. What is available? 6% Lactose solution Goat Milk Cow Milk Lactase enzyme 1 M SPG buffer pH 4 and pH 10 water baths 0,20, 40, 60, 80 C 5M NaCl - Dish soap - deionized water Sucrose Maltose 6% Glucose What do you want in your final solutions? 52% lactose 0.1-0.2 M SPG buffer- for a consistent pH 5-15 ml total volume 0.5-1 ml enzyme solution (pill dissolved in 50 mls H20) a