The enzyme fumarase catalyzes the conversion of fumarate to malate according to OOC H 1.0 fumarate...
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The enzyme fumarase catalyzes the conversion of fumarate to malate according to OOC H 1.0 fumarate 5.0 COO ↑ H ↑ Number 7 H₂O Number OOC- Use the data in the table above to determine the values of Rmax (the maximum rate of the enzyme, often called the maximum velocity, Vmax) and KM (the Michaelis-Menten constant of the enzyme). Answer the three parts below. 0 H H a) If you were to plot this data to graphically determine Rmax and KM using a Lineweaver-Burk plot, what would you plot? If you would use the original data, enter the original value in the corresponding blank. [S] (umol-L-1) Graph as R(umol-L-¹. s-¹) OH H malate -COO [S] (umol-L-1) 1.0 2.0 5.0 2.6 1.0 × 101 20.0 7.2 Graph as Number R (μmol-L-¹-s-¹1) 2.6 4.3 7.2 9.3 10.8 10 Number 0 The enzyme fumarase catalyzes the conversion of fumarate to malate according to OOC H 1.0 fumarate 5.0 COO ↑ H ↑ Number 7 H₂O Number OOC- Use the data in the table above to determine the values of Rmax (the maximum rate of the enzyme, often called the maximum velocity, Vmax) and KM (the Michaelis-Menten constant of the enzyme). Answer the three parts below. 0 H H a) If you were to plot this data to graphically determine Rmax and KM using a Lineweaver-Burk plot, what would you plot? If you would use the original data, enter the original value in the corresponding blank. [S] (umol-L-1) Graph as R(umol-L-¹. s-¹) OH H malate -COO [S] (umol-L-1) 1.0 2.0 5.0 2.6 1.0 × 101 20.0 7.2 Graph as Number R (μmol-L-¹-s-¹1) 2.6 4.3 7.2 9.3 10.8 10 Number 0
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This is MichaelisMentes Plot Graph your data and find the Vmax then in half way Vma... View the full answer
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