3. For each of the three experiments: d) List the methods you used to conduct the...
Fantastic news! We've Found the answer you've been seeking!
Question:
Transcribed Image Text:
3. For each of the three experiments: d) List the methods you used to conduct the experiment (sometimes it will be several, like cell transfection, followed by immunoprecipitation, and western blot). Remember to show the same experimental outcome with two different methods or with pharmacologic (drug) and genetic (SİRNA or CRISPR or gene overexpression) ways to alter protein activity/expression. You can cluster those under the same "experiment." Prioritize "cause and consequence" experiments over "correlative". Make one of the three experiments in vivo (in animals). a) List your experimental groups, including the controls (what do you compare to what? No explanation needed). If you are going to use chemical treatments, indicate tentative concentrations and treatment time. If you are using antibodies, state what they recognize. b) What are the expected results if the hypothesis is right, e.g. after protein X was knocked down/overexpressed/inhibited, metastasis to the lungs increased/decreased over control (specify the control). 3. For each of the three experiments: d) List the methods you used to conduct the experiment (sometimes it will be several, like cell transfection, followed by immunoprecipitation, and western blot). Remember to show the same experimental outcome with two different methods or with pharmacologic (drug) and genetic (SİRNA or CRISPR or gene overexpression) ways to alter protein activity/expression. You can cluster those under the same "experiment." Prioritize "cause and consequence" experiments over "correlative". Make one of the three experiments in vivo (in animals). a) List your experimental groups, including the controls (what do you compare to what? No explanation needed). If you are going to use chemical treatments, indicate tentative concentrations and treatment time. If you are using antibodies, state what they recognize. b) What are the expected results if the hypothesis is right, e.g. after protein X was knocked down/overexpressed/inhibited, metastasis to the lungs increased/decreased over control (specify the control).
Expert Answer:
Related Book For
Financial Statement Analysis
ISBN: 978-0078110962
11th edition
Authors: K. R. Subramanyam, John Wild
Posted Date:
Students also viewed these biology questions
-
For each of the three items, (1) Depreciation, (2) Inventory, and (3) Installment sales, explain: a. Two acceptable accounting methods for reporting purposes. b. How each of the two acceptable...
-
For each of the three income levels shown, pro vide appropriate data to satisfy or be consistent with the absolute and relative income hypotheses. CONSUMPTION MPC ABSOLUTE RELATIVE ABSOLUTE RELATME...
-
For each of the three income levels shown, pro vide appropriate data to satisfy or be consistent with the absolute and relative income hypotheses. SAVING MPS ABSOLUTE RELATIVE ABSOLUTE RELATME INCOME...
-
Suppose that one worker can shovel snow from a storefront sidewalk in 50 minutes and another worker can shovel it in 30 minutes. How long will it take if they work together?
-
A company uses an average of 85 circuit boards a day in an assembly process. The standard deviation is 5 units per day. Tire person who orders the boards follows this rule: Order when the amount on...
-
The Willis Tower in Chicago is 1454 feet tall and is situated about 1 mile inland from the shore of Lake Michigan, as indicated in the figure on the following page. An observer in a pleasure boat on...
-
Identifying the Population In a Gallup poll of 1010 adults in the United States, 55% of the respondents said that they used local TV stations daily as a source of news. Is the 1010 value a statistic...
-
La Jolla Beverage Products is considering producing a wine cooler that would be a blend of a white wine, a ros wine, and fruit juice. To meet taste specifications, the wine cooler must consist of at...
-
How has the role of HRM changed in recent years? Discuss three trends that are changing the HRM function. Why have these roles changed?
-
Outline a long-term approach that you would recommend for narrowing a credibility gap that might exist between an organizations Human Resources department and its department managers.
-
Choose one of the following questions - clearly state which questions you are answering. For each question, apply only 4 of the 5 ethical theories ( Kantianism, Act Utilitarianism, Rule...
-
A piston-cylinder assembly contains 0.5 kg of water at 50C and 500 kPa. It is then isobarically heated until all the water is vaporized. What is the fi nal temperature and volume?
-
Of 500 students in a high school, 72 % have indicated that they are interested in attending college. What is the probability of selecting a random sample of 50 students wherein the sample proportion...
-
Assume that the total value of the following items is $600 billion in a specific year for Upper Mongoose: net exports = $50 billion; value of new goods and services produced in the underground...
-
W is a normally distributed random variable with mean 0 and variance 1, and V is a w2-distributed random variable with degrees of freedom (n - 1). How can both t and F distributions be defined in...
-
Assume that the amount of milk in a 16-oz bottle follows a normal distribution with a mean of 16 and a standard deviation of 1. A consumer protection agency bought 30 bottles of milk and weighed...
-
The previous task of Capstone project 1 9 is connected to this task,please refer to it , and solve this one. Compulsory Task 1 Follow these steps: - Create a copy of your previous Capstone project [...
-
Show that the peak of the black body spectrum as a function of ? is given by eq. (22.14) kg T Wmax = 2.82
-
What factors (limitations) enter into our evaluation of return on net operating assets?
-
Why must income used in computing return on invested capital be adjusted to reflect the capital base (denominator) used in the computation?
-
Why is interest expense ignored when computing return on net operating assets (RNOA)?
-
Cruz Manufacturing Ltds sales slumped badly in 2019. For the first time in its history, it operated at a loss. The companys income statement showed the following results from selling 600 000 units of...
-
The following are emails from various students to Dr. Destiny Sands, who is a professor in the English Department. These students are wondering if Dr. Sands would let them register for her...
-
What are some flawed assumptions about workplace communication? What is the reality for each myth?
Study smarter with the SolutionInn App