*Note, given the data: The fresh media was introduced, and the product was taken out from the system at a rate of 2.1 g/h. The S₀= 1.4 g/L. The density of substrate = 1.56 g/cm3.

QUESTION:

1. Determine the value of Ks, max, maximum dilution rate, and Y_X/S. Evaluate your data.

2. Dilution rate can also be calculated by D = F/V, which describes the relationship between the flow of the media into the bioreactor (F), and the culture volume within the bioreactor (V). Calculate D using this approach and compare with the D value that you have calculated using specific growth rate. Comment on the result.

3. Give an example of production using continuous fermentation. Explain in detail the substrate being used, the product produced, and the system used. Please cite a proper reference