O Attempt 2 Question 7 of 18 To assist in completing this question, you may reference...

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O Attempt 2 Question 7 of 18 To assist in completing this question, you may reference the Animated Technique Video - Gel Electrophoresis (SDS PAGE). A protein sample complex consists of two proteins, a smaller protein, X, and a larger protein, Y. Protein X is composed of two polypeptide chains linked by disulfide bonds. Protein Y is composed of three polypeptide chains linked by disulfide bonds. The complex is analyzed by native PAGE, reducing SDS-PAGE and non-reducing SDS-PAGE. Native PAGE does not include sodium dodecyl sulfate, or SDS. Reducing SDS-PAGE uses both SDS and a reducing agent in the buffer. Non-reducing SDS-PAGE uses SDS, but no reducing agent. Match each gel pattern to an experimental method. The first lane in each gel is a molecular weight ladder. Native PAGE Non-reducing SDS-PAGE Reducing SDS-PAGE 1000 kDa 250 kDa 250 kDa 500 kDa 150 kDa 150 kDa 250 kDa 100 kDa 100 kDa 100 kDa 60 kDa 60 kDa 50 kDa 50 kDa 50 kDa 30 kDa 30 kDa 30 kDa Answer Bank 250 kDa 100 kDa 100 kDa 100 kDa 60 kDa 60 kDa 50 kDa 50 kDa 50 kDa 30 kDa 30 kDa 30 kDa Answer Bank What are the molecular weights of the protein X polypeptides? 60 kDa and 30 kDa Incorrect What are the molecular weights of the protein Y polypeptides? 60 kDa, 50 kDa, and 30 kDa Incorrect O Attempt 2 Question 7 of 18 To assist in completing this question, you may reference the Animated Technique Video - Gel Electrophoresis (SDS PAGE). A protein sample complex consists of two proteins, a smaller protein, X, and a larger protein, Y. Protein X is composed of two polypeptide chains linked by disulfide bonds. Protein Y is composed of three polypeptide chains linked by disulfide bonds. The complex is analyzed by native PAGE, reducing SDS-PAGE and non-reducing SDS-PAGE. Native PAGE does not include sodium dodecyl sulfate, or SDS. Reducing SDS-PAGE uses both SDS and a reducing agent in the buffer. Non-reducing SDS-PAGE uses SDS, but no reducing agent. Match each gel pattern to an experimental method. The first lane in each gel is a molecular weight ladder. Native PAGE Non-reducing SDS-PAGE Reducing SDS-PAGE 1000 kDa 250 kDa 250 kDa 500 kDa 150 kDa 150 kDa 250 kDa 100 kDa 100 kDa 100 kDa 60 kDa 60 kDa 50 kDa 50 kDa 50 kDa 30 kDa 30 kDa 30 kDa Answer Bank 250 kDa 100 kDa 100 kDa 100 kDa 60 kDa 60 kDa 50 kDa 50 kDa 50 kDa 30 kDa 30 kDa 30 kDa Answer Bank What are the molecular weights of the protein X polypeptides? 60 kDa and 30 kDa Incorrect What are the molecular weights of the protein Y polypeptides? 60 kDa, 50 kDa, and 30 kDa Incorrect

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this Answer SDB NonReduing Native PAGE PAGE Reducing SosPAGE 1000 KDa 250KDA 250KDE 500 KD 2... View the full answer