Supercritical fluid chromatography (eng. supercritical fluid chromatography, SFC) uses man carbon dioxide/methanol in the mobile phase (90/10) and the column is a packed column with polar 5 μm silica gel particles. When testing the system by injecting a test substance for you a well-shaped top with a retention factor of 10. You have the back pressure regulator set to 100 bar. If you now make an identical injection afterward but first, the pressure of the backpressure regulator increases to 150 bar, but keeping everything else like flow rate, etc. constant, how does it probably change the retention factor of the test substance? Increases or decreases it and why.

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The retention factor would increase because th... View the full answer