Question 5 An effect of a novel inhibitor was tested on an enzyme and the following...
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Question 5 An effect of a novel inhibitor was tested on an enzyme and the following kinetics were generated: 1 [Substrate] (mm) 3 5 7 9 11 Vo 3.78 5.60 7.27 8.01 9.75 Velocity (mM.min-¹) Vi 2.66 5.12 6.08 7.01 8.52 [SCROLL DOWN] Draw a Lineweaver Burk plot using the data provided (Hint: there should be two plots on your graph) and determine the Km and Vmax value of the non-inhibitor (Vo) and inhibitor (V) [20] a) By analysing the graph, is the inhibitor a competitive or non-competitive? Explain (15) (5) Question 5 An effect of a novel inhibitor was tested on an enzyme and the following kinetics were generated: 1 [Substrate] (mm) 3 5 7 9 11 Vo 3.78 5.60 7.27 8.01 9.75 Velocity (mM.min-¹) Vi 2.66 5.12 6.08 7.01 8.52 [SCROLL DOWN] Draw a Lineweaver Burk plot using the data provided (Hint: there should be two plots on your graph) and determine the Km and Vmax value of the non-inhibitor (Vo) and inhibitor (V) [20] a) By analysing the graph, is the inhibitor a competitive or non-competitive? Explain (15) (5)
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Related Book For
Intermediate Accounting
ISBN: 978-0078025839
9th edition
Authors: J. David Spiceland, James Sepe , Mark Nelson , Wayne Thomas
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