Oxidation of Tritiated Palmitate Palmitate uniformly labeled with tritium (H) to a specific activ- ity of...
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Oxidation of Tritiated Palmitate Palmitate uniformly labeled with tritium (H) to a specific activ- ity of 2.48 x 10 counts per minute (cpm) per micromole of palmitate is added to a mitochondrial preparation that oxidizes it to acetyl-CoA. The acetyl-CoA is isolated and hydrolyzed to acetate. The specific activity of the isolated acetate is 1.00 x 107 cpm/mol. Is this result consistent with the B-oxidation pathway? Explain. What is the final fate of the removed tritium? Oxidation of Tritiated Palmitate Palmitate uniformly labeled with tritium (H) to a specific activ- ity of 2.48 x 10 counts per minute (cpm) per micromole of palmitate is added to a mitochondrial preparation that oxidizes it to acetyl-CoA. The acetyl-CoA is isolated and hydrolyzed to acetate. The specific activity of the isolated acetate is 1.00 x 107 cpm/mol. Is this result consistent with the B-oxidation pathway? Explain. What is the final fate of the removed tritium?
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