1. Flood the starch agar plate with the lodine solution, allow the iodine to remain in contact with the medium for 30 seconds, and pour off the excess. 2. Examine the cultures for the presence or absence of a blue-black color surrounding the growth of each test organism. Describe the Appearance of the medium around the cells Result (+ or -) for Starch Hydrolysis by Polysaccharidases Bacterial species E col P. fluorescens B. subtilis Milk Casein Hydrolysis Examine the milk agar plate cultures for the presence or absence of a clear area, or zone of proteolysis, surrounding the growth of each test organism. Result (+ or -) for Casein Hydrolysis Bacterial Describe the Appearance of species the medium around the cells by Proteases E.coli P. fluorescens B. subtilis 105 P. fluorescens E. coli Bacillus Note: Upper left is the starch, right is the tributyrin, and bottom is the casein. 3. Complete the Review Questions on the back of the worksheet. Your lab quiz will be based on this assignment, so make sure you understand these questions.