Pass years questions. Try it yourself. 1. The diagram below shows a flow sheet for the production and purification of recombinant human serum albumin (rHSA) using expanded-bed adsorption (EBA). In this process, glycerol and methanol are provided as carbon source while ammonia as nitrogen source to promote the growth for P. pastoris. The cells are used as expression host to produce IHSA. In the first medium tank (P-1), 4.27 tons of glycerol and 13 m3 of water are mixed; in the second tank (P-2), 7.4 tons of methanol and 9.4 m of water. Other media components such as salts, vitamins and trace elements are also added to these tanks. A 20% ammonium solution containing 500kg of ammonia is fed in during the fermentation in the bioreactor (P-3). For safety reason, the US made-bioreactor P-3 can only withstand maximum weight 97,885 1b. It is also know that the ratio of salts and vitamins mixture for vessel P-1 is double than P-2. (15 marks) S-009 , 3-001 5-104 P-47 ST-101 5-002 Heat Sterization S-009 P-22/ AF-102 Air Fran wash club 2.1 /V-101 Medium (GM rea Ammonium S-102 epult Meithre Wat Dant S-600 S-007 P-S/ST 102 3.004 Heat Siration P-3/V100 S-006 Fermentation Heating HAC S.101 P.8/V-104 Dilution and pH Adjustment S-105 P-9/C-101 Expanded Bed Adsortion (EBA) State Bufer P-10/V-105 Heat Treatment P-2/V-102 Medium (Mathi S-205 8-202 S 200 Air 8.000 P-7/AF 101 Air Filtration Buller 3 S-200 S-204 5-210 S-206 S-213 5-211 P-6/6-101 Gas Compression S-207 S-214 P-14/OF-101 Disttration (butter ching S212 P-13/C-103 Anion Exchange P-12/7.100 Buffer 2 s-200 P-11/0-102 Hydrophobic chromatography co Storage P-15/V-107 8-216 S-217 8-215 S-218 S-302 S-107 S-219 S-101 S-300 S-904 Stab S 306 S 220 P-16/0-104 Chelate Resin Treatment P.17/UF-102-10 P-19 /V-108 Ultrason X P-18/UF-103 Concentration e Treatment P-20/ DE-101 s.305 P-21 FDR-10 Steril Fitration Freeze Drying (a). In your opinion, where is the portion for feed preparation, reaction and recovery? Draw and indicate clearly in the above flow sheet. (b). Unit operation P-7 and P-22 are both air filtration unit, what is the different between them? (C). Find what is the amount of salts+vitamins mixture used in P-1 and P-2. State all your assumptions