Question: Please answer the following questions using the given information. an amino acid sequence of a heptapeptide gave the following information: Asp, Ala, Lys , Met,

Please answer the following questions using the given information.
an amino acid sequence of a heptapeptide gave the following information: Asp, Ala, Lys, Met, Phe, and Tyr.
1.Trypsin had no effect
2.PITC analysis gave the phenyl alanine derivative
3.Chymotrypsin (which cleaves after aromatic amino acids) gave a dipeptide and tetrapeptide, which contained Leu, Lys and Met.
4. Cyanogen bromide, which cleaves after methionine residues, gave a dipeptide, tetrapeptide, and free lys.
Question 1.Trypsin had no effect on the polypeptide what does this tell you about the sequence N and C terminus?
Question 2. PITC analysis gave the phenyl alanine derivative what does this tell you about the N and C terminus?
Question 3. please show the correct cleavages of each step
Question 4. Using the information above what is the new final sequence?

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