Please answer the questions on the bottom by creating graphs and charts relating to this sample data. Thank you so much!

1. (4 pts) Construct a figure using your picture of your gel. Annotate the gel by numbering the lanes, marking the ladder, and identifying/labeling sample bands on the sides of the gel. Be sure to also include an adequately detailed figure legend.

2. (4 pts) Calculate the R_f values of the bands in the molecular weight standard and the protein bands observed in one of the mixed protein sample lanes. Construct a table. For the bands from the standard (table 2), include: R_f, molecular weight (MW), and log (MW). For the bands from the mixed protein sample lane (table 3), include: R_f, calculated log (MW), calculated MW, visually estimated MW, and predicted band identity. You can present this data as two separate tables if you would like.

3. (4 pts) Create a figure (Figure 2) showing the relationship between the log (MW) of each standard and its corresponding R_f value, log (MW) vs. R_f (R_f on the x-axis). Also, create a figure (Figure 3) showing the relationship between the molecular weight of each standard and its corresponding R_f value, R_f vs. MW (R_f on the y-axis. Note that the axes are switched for these two plots). Both figures should be scatter plots with a trend line, equation, axis labels, and legend.

all the information is provided in the first picture! Thank you!

Below is the sample data you will use. Information for the legend: Note that the rightmost 4 lanes all contain the same protein mixture just different amounts. There are five bands in each sample protein Lane (1-4) that can be analyzed. Note that the blue band along the very bottom of the gel reduces in intensity Lane 1 Lane 4. This indicates that this is a protein band not the dye front, since the same amount of dye solution was loaded in each lane. This band is running just a little bit slower than the dye front. Assume the dye front is the same as the bottom of the image. Lane 1. 7.5 pg protein, lane 2. 5 ug protein. Lane 3. 2.5 ug protein. Lane 4. 1.25 ug protein. Lane five. 7 UL Promega ladder. The numbers are in kilodaltons (kd). - 150 -100 75 50 35 25