PLEASE EXPLAIN THIS VERY CLEARLY IN A WAY A FIRST TIME LEARNER WOULD UNDERSTAND EACH STEP BY STEP PROCESS, WRITE IT OUT AND EXPLAIN ARTICULATELY PLEASE.

PART A:

An enzyme-catalyzed reaction has a given catalytic power, X. A mutant form of the enzyme has a catalytic power that is equal to 0.85X. What might you hypothesize about why the mutant has decreased catalytic power? Suggest at least 2 possibilities based upon how you understand enzymes catalyze reactions.

PART B:

Acetylcholinesterase catalyzes the breakdown of the neurotransmitter acetylcholine to choline and acetate. The following kinetic data were obtained in a kinetic assay of acetylcholinesterase. Estimate the Km and Vmax of the enzyme.

[acetylcholine] (M)	Vo (V naut) (M/min)
1	25
4	81
16	213
44	317
105	382