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Bacterial Transformation Lab Extension BIO 121 Scientific Reasoning Assessment Spring 2024 Part1 DNA transformation in bacteria involves important components, such as plasmids, bacterial competence, and transformational efficiency. Define a plasmid. Define bacterial competence. Describe transformational efficiency. Cite TWO sources that provide background information about plasmids. Cite TWO sources that provide background information about bacterial competence. Cite TWO sources that discuss transformational efficiency. Part2 Inan experiment, plasmid pSALF was isolated and purified in preparation for transformation. In addition, three E. col strains are available: lacZ mutant (LZM), wild-type-K-12 strain (WK12), and iron-uptake mutant (IUM) 2. Using the information about plasmids and bacterial competency, pose a question you have about transformation efficiency. b. State a hypothesis about transformation efficiency. Design an experiment to test your hypothesis You have available to you: Plasmid pSALF with the gene for ampicillin resistance. ii. Agar plates Competent strains of the above E. coli cells and all materials needed for bacterial transformation. Using the materials above and other appropriate laboratory techniques discussed in BIO 121, write a concise list of steps to design an experiment to determine the transformation efficiency of bacteria with the above plasmid. You do not need to include every detail aboat your laboratory technique; however, be sure to include the appropriate controls. At the end of your list, make a prediction about the results of your experiment. Part3 You will now simulate transformation efficiency using a random number generator. Go to www.random.org. Generate a random number for between 1 and 100. Do this for each below and record these numbers: First random number: (use this number for the level of transformation efficiency for LZM) Second random number: (use this number for the level of transformation efficiency for WK12) (use this number for the level of 'Third random number _ transformation efficiency for [UM) Part4 Using the numbers above, draw a bar chart below. a. Give your chart an appropriate title. What s your independent variable? Give the x-axis an appropriate label. What s your dependent variable? Give the y-axis appropriate label Draw and label bars for the number of colonies using the numbers generated in Part What would you expect the number of colonies to be for a negative control? What would you expect the number of colonies to be for a positive control? Draw and label bars for what you would expect for your control: h. Write a summary statement about your results below. Title: Part5 Discuss your results above. Are these the results you expected? What would be an appropriate follow up experiment