Please Help! thank you so much

1. Preparing an inoculum a. You made a 10 ml overnight culture of yourfavorite pathogen and it grew very well. After using a spectrophotometer, you find that the culture density is 9x109 cells/mL. But, for your experiment, you need a 10 mL inoculum of 4.5x103 cells/mL. Use the C1V1=02V2 equation to see how many mL of culture you need and how many mL of broth you need to prepare the culture for your experiment. Show your work. (1 pt) C1 = 02: V1 = V2: b. Your smallest pipettor is a p10 with a volume range of 1 ul 10 ul. Based on your answer from part A, can you accurately pipette this volume? If not, how would you dilute the overnight culture to prepare the inoculum? Use a short paragraph or drawings to illustrate your method. (1.5 pts) 2. Setting up a PCR reaction a. You extracted DNA from your pathogen and need to determine the concentration. You think you obtained a high amount of DNA, so you decide to do three 10-fold dilutions before taking an absorbance reading. The reading is 0.5 ng/jL. What is the actual concentration of the DNA you extracted? (1 pt) b. You need to amplify a certain gene from your DNA using PCR. You decide to set up a 50 uL reaction containing the components in the table below. The reaction requires 25 ng of DNA. You dilute your DNA to 5 ng/JL. How many UL of DNA should you add to the reaction? How many UL of water? Show your work. (1.5 pts) Component Volume in tube GoTaq@ master mix 25.0 UL ddH20 23.0 UL - X UL Primer (forward) 1.0 UL Primer (reverse) 1.0 UL Template DNA X uL (25 ng) Total Volume 50.0 uL