Question: Please I need help, I dont have much understanding for the softwares i need to use for my term paper. My protein name is Phosphoenolpyruvate

Please I need help, I dont have much understanding for the softwares i need to use for my term paper.

My protein name is Phosphoenolpyruvate protein phosphotransferase.

I am to Get the protein structure and upload it to Swiss pdb viewer. And describe the important areas of the protein including substrate binding sites and other important residues.

Blastp alignments: Use blastp to obtain proteins that are 90,80, 70, 60 and 50% similar. You may needto get the first

1000 or 5000 results so you do not get 100% sequence identity.

Align the sequences in Bioedit. Identify and discuss the conserved regions and any residues that have been shown to be in the active site or any residues that involve binding to inhibitors, other protein or are of interest. There are many cases in the literature where site mutations have demonstrated the residues importance.

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