Question: PLEASE PLEASE HELP! Please answer all parts!! thank you 3. How much does serum cost? Compare the price of calf serum (CS), fetal bovine serum
3. How much does serum cost? Compare the price of calf serum (CS), fetal bovine serum (FBS), horse serum (HS), and iron supplemented calf serum (ISCS). 4. Complete the cost table for the amount of serum required to prepare 1L of medium containing one of the following: Cost/unit (e.g. Total cost/1L Serum Vendor per 100 ml or medium 500 ml) 10% FBS 5% FBS 10% CS 5% CS 5% CS; 5% FBS 10% HS 5% HS 5% HS; 5% FBS 10% ISCS 5% ISCS Suggested vendors: Thermo Fisher Scientific, Sigma, VWR. The rate at which cells grow in culture depends on factors intrinsic to the particular cells as well as extrinsic factors including nutrients supplied in the media. For part 2 of this module, you will investigate the influence that the amount and type of serum have on the proliferation rate of 3T3 cells. Please refer to the following sources to complete question set six and develop an experimental plan. Your lecture notes Textbook reading: Chapters 8 and 9.1-9.3 (7ed: pp. 125-145, 149-155). Pubmed searches Protocols posted on Blackboard Question Set 6 1. What is serum? How is it isolated? What nutrients does it provide to cells? 2. What are some disadvantages of supplementing media with serum? 3. How much does serum cost? Compare the price of calf serum (CS), fetal bovine serum (FBS). horse serum (HS), and iron supplemented calf Serum (ISCS). 4. Complete the cost table for the amount of serum required to prepare 1L of medium containing one of the following: Serum Vendor Cost/unit (e.g. per 100 ml or 500 ml) Total cost/1L medium 10% FBS 5% FBS 10% CS 5% CS 5% CS: 5% FBS 10% HS 5% HS 5% HS: 5% FBS 10% ISCS 5% ISCS Suggested vendors: Thermo Fisher Scientific Sigma, VWR 5. From a cost only perspective, which serum would you select? . Your instructor will help you select sera to test effectiveness. Illustrate your experimental plan, 3. How much does serum cost? Compare the price of calf serum (CS), fetal bovine serum (FBS), horse serum (HS), and iron supplemented calf serum (ISCS). 4. Complete the cost table for the amount of serum required to prepare 1L of medium containing one of the following: Cost/unit (e.g. Total cost/1L Serum Vendor per 100 ml or medium 500 ml) 10% FBS 5% FBS 10% CS 5% CS 5% CS; 5% FBS 10% HS 5% HS 5% HS; 5% FBS 10% ISCS 5% ISCS Suggested vendors: Thermo Fisher Scientific, Sigma, VWR. The rate at which cells grow in culture depends on factors intrinsic to the particular cells as well as extrinsic factors including nutrients supplied in the media. For part 2 of this module, you will investigate the influence that the amount and type of serum have on the proliferation rate of 3T3 cells. Please refer to the following sources to complete question set six and develop an experimental plan. Your lecture notes Textbook reading: Chapters 8 and 9.1-9.3 (7ed: pp. 125-145, 149-155). Pubmed searches Protocols posted on Blackboard Question Set 6 1. What is serum? How is it isolated? What nutrients does it provide to cells? 2. What are some disadvantages of supplementing media with serum? 3. How much does serum cost? Compare the price of calf serum (CS), fetal bovine serum (FBS). horse serum (HS), and iron supplemented calf Serum (ISCS). 4. Complete the cost table for the amount of serum required to prepare 1L of medium containing one of the following: Serum Vendor Cost/unit (e.g. per 100 ml or 500 ml) Total cost/1L medium 10% FBS 5% FBS 10% CS 5% CS 5% CS: 5% FBS 10% HS 5% HS 5% HS: 5% FBS 10% ISCS 5% ISCS Suggested vendors: Thermo Fisher Scientific Sigma, VWR 5. From a cost only perspective, which serum would you select? . Your instructor will help you select sera to test effectiveness. Illustrate your experimental plan
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